A novel method of combining Periodic Acid Schiff staining with Wright-Giemsa staining to identify the pathogens Penicillium marneffei, Histoplasma capsulatum, Mucor and Leishmania donovani in bone marrow smears
نویسندگان
چکیده
منابع مشابه
Fluorescent staining of Histoplasma capsulatum.
In an earlier paper from this laboratory (Gordon, 1958) it was demonstrated that fluorescein-labeled specific antiserum employed as a cellular stain will differentiate Candida albicans and Candida tropicalis from other yeasts and yeastlike organisms, including the remaining members of the genus Candida. The present report describes the preparation and use of a similar reagent for detection and ...
متن کاملI. Histochemical Staining of Bone Marrow Smears
Histochemical procedures for PMN granule enzymes were carried out on smears prepared from normal rabbit bone marrow, and the smears were examined by light microscopy. For each of the enzymes tested, azo dye and heavy metal techniques were utilized when possible. The distribution and intensity of each reaction were compared to the distribution of azurophil and specific granules in developing PMN...
متن کاملIntroducing a combined Leishman-Giemsa stain as a new staining technique for avian blood smears
Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...
متن کاملBone marrow aspirate showing Histoplasma capsulatum.
A 48-year-old male with history of alcohol abuse and untreated HIV presented with 1-week history of fever, abdominal pain, and diarrhea. Complete blood counts showed pancytopenia with hemoglobin of 71 g/L, total leukocyte count of 2.2 3 10/L, and platelet count of 92 3 10/L. Initial renal, liver, and coagulation studies were normal. CD4 count was 23/mL, and HIV viral load was 3 600 000/mL. Hepa...
متن کاملComparison of staining methods and a nested PCR assay to detect Histoplasma capsulatum in tissue sections.
To optimize diagnosis of histoplasmosis in tissue sections, 30 spleen specimens from mice, experimentally infected with Histoplasma capsulatum, were examined by H&E, Grocott stain, anti-bacille Calmette-Guerin antibody immunostain, Fungiqual A fluorochrome stain (Drs Reinehr and Rembold, Kandern, Germany), and a nested polymerase chain reaction (PCR) assay. Results were compared with the tissue...
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ژورنال
عنوان ژورنال: Experimental and Therapeutic Medicine
سال: 2015
ISSN: 1792-0981,1792-1015
DOI: 10.3892/etm.2015.2357